The digestion reaction of ChpBK was conducted in a 20-μl reaction mixture with 10 pmol of RNA oligo (RB1), 1 μg of ChpBK, 20 U of RNase inhibitor, and 10 mM tris-HCl buffer (pH 7.5). The mixture was incubated at 37°C for 30 min. The digestion reaction of ChpBK with different m6A percentage was conducted as described above. The MazF-K56A cleavage reaction was performed in a 20-μl reaction mixture with 10 pmol of RNA oligo, 2 μg of MazF-K56A, and 1× MazF buffer [40 mM sodium phosphate (pH 7.5) and 0.01% Tween 20] at 37°C for 30 min. All samples were loaded on the 15% urea polyacrylamide gel and electrophoresed in 0.5× TBE buffer.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.