Prediluted sera (1:40), heat-treated for complement inactivation, from mice receiving HEDGES treatment for heavy and light IgG chain genes of 5J8 (anti-H1) or rituximab (anti-hCD20, a negative control) were tested in triplicate for the capacity to neutralize 100× TCID50 (median tissue culture infectious dose) Cal09 H1N1 IAV (strain X-179a) in a standard in vitro microneutralization assay (36). Data were normalized by setting control serum to 0% and uninfected wells to 100%.

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