Plasma levels of hG-CSF were analyzed using the hG-CSF Quantikine enzyme-linked immunosorbent assay (ELISA) kit (SCS50; R&D Systems). Serum rituximab levels were analyzed using the rituximab ELISA kit (IG-AB106; Eagle Biosciences). For anti–hG-CSF, 5J8, and anti–IL-5 mAb ELISAs, immunoassay plates were coated with antigen (BioLegend 578606 and Sino Biologicals 11085-V08H and 15673-HNCE, respectively) and blocked with 3% bovine serum albumin. After binding of expressed serum proteins, detection was carried out with enzyme-linked anti-human IgG and 3,3′,5,5′-tetramethylbenzidine substrate (AP113P; Millipore). For the anti-rituximab ELISA, the coat protein was rituximab from InvivoGen, and the detection antibody was goat anti-mouse IgG H+L horseradish peroxidase from Thermo Fisher.

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