Mice were euthanized via CO2 inhalation, and blood was cleared by perfusion of phosphate-buffered saline. For flow cytometry, lungs were harvested and minced, and then frozen in CELLBANKER 2 freezing medium. For histopathology, organs were harvested and fixed in 10% neutral buffered formalin before transfer into 70% ethanol for staining and analysis at the University of Washington Histology and Imaging Core.

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