Two micrograms of each genomic DNA was converted with sodium bisulfite using the EZ DNA Methylation Gold Kit (Zymo Research), according to the manufacturer’s instruction. Bisulfite-modified DNAs were amplified using target-specific primers (table S4). For sequence analysis, the polymerase chain reaction (PCR) products obtained after bisulfite conversion were purified using QIAquick PCR Purification Kit (Qiagen) and were then ligated into the pCR4 TA cloning vector (Thermo Fisher Scientific). Sixteen colonies for each cloned sample were sequenced and evaluated.

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