HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco, 11995-073) supplemented with 10% heat-inactivated fetal bovine serum (Gibco, A3160702), penicillin (100 U/ml), and streptomycin (100 μg/ml). Both fibroblasts and LCLs including controls and those from patients with the Pro370Ser and Arg455* variants were prepared as described (12). Breast cancer MCF7 cells were cultured in the above medium. MEFs were prepared and cultured as previously described (12). MEFs from tamoxifen-inducible Brpf1ff;ER-Cre embryos were used to obtain Brpf1-null MEFs after treatment with 0.25 μM 4-hydroxytamoxifen (Sigma-Aldrich, T176) for 3 days in vitro (39). Kat6a-null MEFs were prepared from Kat6af/f;EIIa-Cre embryos as described (57).

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