Mice strains were maintained in the Comparative Medicine and Animal Resources Centre of McGill University, Montreal, Quebec, Canada. All procedures related to mouse work were performed according to an animal use protocol approved by the McGill University Animal Care Committee.

Brpf1f/f mice were maintained in C57BL/6J genetic background, in which the conditional Brpf1f allele contains two LoxP sites flanking exons 4 to 6 (39, 55, 56). Breeding with Brpf1f/f mice and UBC-Cre/ERT2 strain (The Jackson Laboratory, 007001) generated Brpf1f/+;ER-Cre mice, which were further intercrossed to yield Brpf1f/f;ER-Cre embryos for MEF isolation (39). For epiblast-specific knockouts, Brpf1f/f mice were crossed with the Meox2-Cre strain (The Jackson Laboratory, 003755). Brpf1 knockout embryos (E10.5) were prepared from intercross of Brpf1f/+;Meox2-Cre mice, as previously described (12). The Kat6af/f mice line was generated in a mixed C57BL/6J-CD1 genetic background (12). The Kat6af allele contains two LoxP sites flanking the second coding exon. Crossing this mice line with the EIIa-Cre stain (The Jackson Laboratory, 003724) yielded Kat6af/+;EIIa-Cre mice, which were further intercrossed to generate Kat6a knockout embryos and MEFs (57). Knockout efficiency was verified by genomic polymerase chain reaction (PCR) and/or reverse transcription quantitative real-time PCR (57).

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