As previously described (10), 5 × 1010 and 2 × 1011 T4 or T4-AAV particles were intramuscularly injected into BALB/c mice. At 0.25, 0.5, 1, 2, 5, 10, 15, 30, and 60 days after administration, 30 μg of RediJect D-Luciferin Ultra (PerkinElmer, MA), a luciferase substrate dissolved in 0.9% saline, was injected intraperitoneally. After 5 min, the mice were lightly anesthetized with 2% isoflurane and placed on an IVIS 200 bioluminescence whole-body imaging workstation (Caliper). The bioluminescence emission signal was quantified using the camera control program, Living Image software, and displayed in physical units of surface radiance, photons per second per centimeter squared per steradian (photons s−1 cm−2 sr−1).

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