The T4-AAV complex was applied to the carbon grid for 5 min at RT. The grid was then frozen in liquid nitrogen using Gatan CP3 cryo-plunger. The cryo–electron microscopy images of T4, AAV, and T4-AAV complex were collected by Q. Fang at Purdue University using a Titan Krios microscope equipped with a charge-coupled device camera. AAV particles for this experiment were provided by M. Agbandje-McKenna, University of Florida.

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