For assembly of T4-Soc-AAV vectors on nickel beads, T4-Soc(His6)-biotin vectors were loaded onto Ni2+-NTA (nitrilotriacetic acid) agarose beads (Qiagen, The Netherlands). After incubation for 1 hour at 4°C, the mixture was centrifuged at 100g for 30 s and washed five times with binding buffer. Avidin dissolved in binding buffer was then added to the beads. After 20 min of incubation at 4°C, free avidin was removed by washing and centrifugation. BioAAV vectors were then added to the T4-Soc-SBA immobilized beads and incubated for 30 min. After washing five times with binding buffer, bound T4-Soc-AAV vectors were eluted with elution buffer [50 mM tris-HCl (pH 8.0), 300 mM NaCl, and 300 mM imidazole]. The protocol for assembling T4-Hoc-AAV on nickel beads was similar to that used for T4-Soc-AAV. Last, the eluted vectors were exchanged into PBS-MK buffer.

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