AAV vectors and Soc proteins were biotinylated using EZ-Link Sulfo-NHS-LC-biotin according to the manufacturer’s instructions (Thermo Fisher Scientific, MA). Purified AAV vectors (1 × 1012 particles) were incubated with 500 nmol of Sulfo-NHS-LC-biotin at 37°C for 2 hours in PBS buffer. For Soc protein biotinylation, a 20-fold molar excess of the biotin reagent was used to label 10 mg of Soc in PBS buffer. To remove free biotin, the reaction mixture was dialyzed against PBS-MK buffer in Slide-A-Lyzer dialysis cassettes (Thermo Fisher Scientific, MA). Dialyzed bioAAV and bioSoc were concentrated and stored at −80°C.

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