MMOH (1.0 μM), MMOB, (2.0 μM) and MMOR (0.5 μM) were added to a 25 mM solution of Mops (pH 7.5) containing DTT (1 mM) and NaCl (10 mM), and propylene gas was bubbled through the mixture (Hankook Special Gas) for 20 min. Subsequently, the mixture was incubated at 25°C. Steady-state kinetics were measured using a Cary 60 UV-visible spectrometer by the addition of NADH at 340 nm (extinction coefficient = 6220 cm−1 M−1). The inhibitory activities of MMOD were measured in the absence or presence of progressively increasing amounts of MMOD. Products were confirmed by gas chromatography (YL 6500 GC system) using an Agilent HP-PLOT Q stationary column (30 m by 0.535 mm by 40.00 μm). Independent experiments were repeated in triplicate to calculate the mean and the SEM.

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