To track the presence of the bacteriocin along the purification, samples were tested for their antimicrobial activity against Cp at each step using the diffusion assay. If needed, samples were concentrated using a Speed-Vac concentrator (Thermo Fisher Scientific). A 10−3 dilution of an overnight culture of Cp strain [American Type Culture Collection (ATCC) 13124] was spread on BHI-YH agar. After drying for 30 min at room temperature, wells with a diameter of 6 mm were dug with a Pasteur pipette and 100 μl of sample was added per well. Alternatively, 10 μl was spotted directly onto the plate. The presence of inhibition halos around the samples was examined after incubation of the plates at 37°C for 24 hours under anaerobic conditions.

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