Gel containing samples was decolorized and washed to make it transparent and then freeze-dried. Samples were reduced of disulfide bond by dithiothreitol and alkylation before enzymatic hydrolysis. Then, the peptide segment was extracted and dried in vacuum. Samples were desalted, and supernatant was added to the sample flask for mass spectrometry (Q Exactive) detection. The MaxQuant search database was retrieved.

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