Breast cancer cells (500 to 800 cells per well) were seeded in 96-well culture plates and cultured for 3, 5, or 7 days. MTT (M-2128, Sigma-Aldrich) was added to reach the final concentration of 0.5 mg/ml, and the cells were incubated at 37°C for 4 hours; then the supernatant was removed, and 100 μl of dimethyl sulfoxide was added. The optical density value was measured at 490 nm after shaking it for 10 min.

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