For sample preparation, when the cell culture reaches to 80% confluence, the cells were collected and lysed for 40 min on ice. Extracted protein samples were denatured in 5× loading buffer containing SDS in boiling water for 10 min. Five percent nonfat dry milk for detection of conventional proteins or 5% bovine serum albumin for detection of phosphorylated proteins dissolved in tris-buffered saline containing 0.1% Tween 20 was used for blocking and antibody dilution. The following antibodies and dilutions were used: MSN (1:2000, ab52490, Abcam), actin (1:1000, HC201, TransGen), FLAG (1:2000, F7425, Sigma-Aldrich), phosphorylated MSN (1:1000, ab177943, Abcam), lamin (1:1000, ab133256, Abcam), tubulin (1:2000, HC101, TransGen), NONO (1:1000, 11058-1-AP, Proteintech), pCREB [1:1000, 87G3, Cell Signaling Technology (CST)], CREB (1:1000, 48H2, CST), pPKCζ (1:1000, 9378S, CST), PKCζ (1:500, sc-17781, Santa Cruz Biotechnology), pPKA (1:1000, 4781, CST), PKA (1:1000, 4782, CST), goat anti-mouse immunoglobulin G (IgG)–horseradish peroxidase (HRP) (1:5000, HS201-01, TransGen), and goat anti-rabbit IgG-HRP (1:5000, HS101-01, TransGen). Western HRP Substrate (WBKLS0500, Millipore) was used to detect HRP-conjugated secondary antibodies. Software (ImageJ) was used to quantify the relative expression of proteins, which was presented as the ratio of test protein integrated density to internal control integrated density. All uncropped images from Western blots displayed in the study are presented in supplementary data (fig. S10).

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