Total RNA was extracted with TRIzol (9109; Takara Bio), and the RNA concentration was measured with NanoDrop (Thermo Scientific, USA). RNA (1 μg) was reverse- transcribed to complementary DNA (cDNA) using the HiScript II 1st Strand cDNA Synthesis Kit (R211-02, Vazyme) and the T100 Thermal Cycler (Bio-Rad). qRT-PCR was carried out to detect the expression level of related genes in this study using AceQ qPCR SYBR Green Master Mix (Q111-02, Vazyme), and it was performed using 7300 Plus Real-Time PCR System (Applied Biosystems). RT-PCR data for mRNA are shown relative to reference gene. The formula of relative expression value was as follows: 2(−ΔCt). The gene-specific primers used are listed below in Table 1.

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