Breast cancer cell lines SUM149 and SUM159 were from Asterland; MCF7, T47D, SKBR3, BT474, HCC1954, and MDA-MB-231 were purchased from the American Type Culture Collection. The culture medium for SUM149 and SUM159 was Ham’s F-12 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), insulin (5 μg/ml), hydrocortisone (1 μg/ml; both from Sigma-Aldrich, St. Louis, USA), and 1% penicillin-streptomycin (pen-strep) (Beyotime, China). The culture medium for MCF7 was Eagle’s minimum essential medium (Gibco, USA) supplemented with 10% FBS, insulin (10 μg/ml), and 1% pen-strep. The culture medium for T47D was RPMI 1640 (Gibco, USA) supplemented with 10% FBS, insulin (5 μg/ml), and 1% pen-strep. The culture medium for SKBR3, BT474, HCC1954, and MDA-MB-231 was RPMI 1640 supplemented with 10% FBS and 1% pen-strep. All the cell lines were tested and authenticated. These cell lines were maintained at 37°C in an atmosphere of 5% CO2.

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