Na+-selective polymeric solution was produced by mixing 30:10:65:0.55:1 weight ratio of PVC, silicone rubber, DOS, Na-TFPB, and Na ionophore X in 660 μl of THF. K+-selective solution was produced by dissolving 47:60:96:1:4 weight ratio of TPU, silicone rubber, DOS, NaTPB, and valinomycin in 700 μl of THF. Na+ (2 μl/mm2)– and K+ (3 μl/mm2)–selective solutions were drop-casted onto the printed carbon electrodes and left to dry overnight under ambient conditions. To fabricate glucose sensors, carbon electrodes were modified by pulse deposition of Au in 0.1 M HAuCl4 between 0 and −2 V for 2000 cycles at 50 Hz. Glucose-selective cocktail was prepared by first dissolving 0.5% agarose in deionized (DI) water using magnetic stirring at 120°C. The agarose solution was then mixed with GOx solution [10 mg/ml in phosphate buffered saline (PBS) (pH 7.2)] at 2:1 (v/v) ratio. A Prussian blue mediator layer was deposited onto the electrodes by cyclic voltammetry from 0 to 0.5 V (versus Ag/AgCl) for one cycle at a scan rate of 20 mV/s in a solution containing 2.5 mM FeCl3, 100 mM KCl, 2.5 mM K3Fe(CN)6, and 100 mM HCl. Three microliters of the glucose-selective cocktail were drop-casted onto the carbon electrodes and left to dry overnight under ambient conditions.

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