The native and engineered RBCs were plated in 96-well plates at 1 × 105 cells per well in 100 μl of 10 mM PBS. Then, 10 μl of anti-D, anti-A, and/or anti-B blood grouping reagent (monoclonal antibodies, SHPBC, China) was added to each well, and the cells were incubated for 1 hour at room temperature. Incubated cells were stirred using pipette tips and then observed using optical microscopy, and the images were captured on an Axio Observer A1 microscope (Zeiss, Germany).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.