Five microliters of CCHFVLP or VEEVLP were applied to formvar-coated 200-mesh nickel grids and incubated for 15 to 20 min. VLP grids were blocked with 4% normal goat serum for 5 min and then wicked dry. Samples were then incubated for 20 to 30 min with either mAb-13G8 (1:500), mAb-11E7 (1:1000), or a negative control antibody H3C8 (1:1000). A control with buffer solution (without primary antibody) was prepared in parallel. Samples were rinsed with buffer for 5 min. Secondary antibody (10-nm gold; goat anti-mouse; 1:25) was added to all samples, incubated for 15 min, washed for 5 min with buffer, then fixed with 1% glutaraldehyde for 1 min, and rinsed in Milli-Q water. Samples were negative stained for 1 min with 1% uranyl acetate and subsequently imaged on a Jeol 1011 transmission electron microscope at various magnifications.

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