Native uricase, PEG-uricase, and PTMAO-uricase conjugates were incubated in PBS solution (pH 7.4) at 10 μg/ml for 6 hours with and without urea (0.4, 0.8, 1.6, and 3.2 M), after which the activities of the retained uricase were tested. Likewise, the thermal stability of native uricase, PEG-uricase, and PTMAO-uricase conjugates were tested after incubation at different temperatures (40°, 50°, 60°, and 70°C) for 30 min. The activity of uricase was measured by AmplexTM Red Uric Acid/Uricase Assay kit following the manufacturer’s protocol. Measurements were performed in triplicate.

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