CPICs were synthesized in our laboratory as follows: (i) For the preparation of solution A, 0.20 g of CaCl2·2H2O and 3.8 ml of TEA were added into 80 ml of ethanol and then ultrasonicated for 5 min. (ii) For the preparation of solution B, 70 μl of H3PO4 was added into 20 ml of ethanol and stirred thoroughly. Solution B was dropped into solution A with slight agitation, and then CPICs (2 mg/ml) formed in the solution. The gel-like CPICs were collected through centrifugation [30,427 relative centrifugal force (Rcf)] and washed twice with ethanol.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.