The nonspecific protein adsorption of the TMAO polymer films was determined with a SPR biosensor using a flow rate of 40 μl/min at 25°C. After first establishing a baseline using PBS, undiluted human serum was flowed for 10 min, followed by buffer to reestablish the baseline. Protein adsorption was quantified as the difference between buffer baselines and converted to a surface coverage using the appropriate sensitivity factor.

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