TMAO hydrogel was fabricated by bulk photopolymerization with a hydrogel aqueous solution containing TMAO monomer (0.67 g of Milli-Q water and 330 mg of TMAO monomer), cross-linker N,N′-methylenebis(acrylamide) [1 weight % (wt %), 3.3 mg], and photoinitiator 2-hydroxy-2-methylpropiophenone (0.33 mg). The hydrogel aqueous solution was placed between two glass slides separated by a 0.5-mm-thick polytetrafluoroethylene spacer and was then photopolymerized at room temperature for 30 min. After polymerization, hydrogels were removed from the casts and soaked in phosphate-buffered saline (PBS) for 3 days to remove unreacted chemicals and to reach the fully hydrated hydrogel network. PBS was refreshed every 12 hours.

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