Huh7 and SW13 cells were propagated in Dulbecco’s modified Eagle’s medium with Earle’s salts (Corning), both medias were supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin/streptomycin (Gibco), 1% sodium pyruvate (Sigma-Aldrich), 1% l-glutamine (HyClone), and 1% Hepes (Gibco). Minimally passaged CCHFV strain Afg09-2990 (39) or strain IbAr 10200 (USAMRIID collection) were used for all experiments, as indicated. Strain Afg09-2990 was previously passaged three times in Vero cells and then propagated at USAMRIID two times in Huh7 cells. Strain IbAr 10200 was passaged, as previously described (25). The viruses were collected from clarified cell culture supernatants and stored at −80°C. All CCHFV work was handled in biosafety level 4 (BSL-4) containment at USAMRIID.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.