Statistical analysis was performed with Prism software (GraphPad Software Inc.). One-way analysis of variance (ANOVA) followed by Bonferroni’s test was used to perform multigroup comparisons of T cell frequency. An unpaired or paired t test was used to compare the T cell responses between pDQ8cis and pDQ8trans. The extra sum-of-squares F test was used to compare EC50 between different DQ8cis/trans proteins.

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