Multiple T1D-associated islet antigens, IA-2, ICA69, Phogrin, and Zn T8, were screened using the DQ8cis binding prediction algorithm in NetMHCIIpan (43) to identify potential DQ8cis/trans-restricted peptides. The top 20 to 25 ranking peptides for each protein were selected for T cell experiments. IGRP-specific epitopes was identified directly by Tetramer Guided Epitope Mapping. Peptides derived from influenza antigens, islet antigens, and a panel of modified GAD65250–266, InsB11–24, and IA-2957–972 peptides with alanine, glycine, or phenylalanine substitution were synthesized by Mimotopes (Clayton, Australia).

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