All manipulations were carried out under argon atmosphere. Commercially available reagents were used as received without purification. Column chromatography was carried out on silica gel (300 to 400 mesh). Analytical thin-layer chromatography (TLC) was performed on glass plates of Silica Gel GF254 with detection by ultraviolet. 1H and 13C nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AVANCE 400 MHz/500 MHz spectrometer with trimethylsilyl (TMS) as reference. Infrared spectra were obtained on an Agilent Cary 630 instrument on a diamond plate using attenuated total reflection. High-resolution MS was conducted on an Agilent 6540 Q-TOF LC-MS equipped with an ESI probe operating in positive ion mode.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.