Passive viewing task. To investigate whether cvGPe and cdlSNr neurons responded to bad objects, we applied a paired t test (two-tailed) to neuronal spike counts. These responses to bad objects (from 100 to 300 ms after bad object onset) were compared with baseline activities (from 200 to 0 ms before bad object onset) in the same trials (Fig. 1, C and D).

Sequential saccade choice task. To investigate the difference in behavioral (saccade) response to good and bad objects, we used a Mann-Whitney U test (two-tailed) for saccade latency (fig. S1) and for saccade choice more than a month after the last session of the task (Fig. 2B). To investigate the neuronal data, we used a paired t test (two-tailed) (Fig. 2, C and D). A total of 55 cvGPe neurons and 52 cdlSNr neurons were used for this analysis, respectively. To investigate whether these cvGPe and cdlSNr neurons responded to bad objects, we compared responses to bad objects (from 100 to 300 ms after bad object onset) with baseline activities (from 200 to 0 ms before bad object onset) in the same trials. To investigate whether these cvGPe and cdlSNr neurons showed different response depending on action choice to bad objects (saccade or not), we compared their activity between saccade (+) trials and saccade (−) trials in the same spike count window (from 100 to 200 ms after bad object onset).

Neuropharmacological experiments. To investigate whether the bicuculline injection affected cdlSNr activity in the sequential saccade choice task, we applied a Mann-Whitney U test (two-tailed) to compare the activity of the same cdlSNr neuron between before and after the injection, separately for good or bad objects (Fig. 3D). To investigate whether the bicuculline injection modulated saccade rate to good or bad objects in the sequential saccade choice task, we applied a Wilcoxon signed-rank test (two-tailed) to compare the saccade rates between before and after bicuculline injection (Fig. 3C and fig. S3A) and saline injection (fig. S3B). To investigate whether the injection modulated the saccade choice rate to bad objects in the simultaneous saccade choice task, we applied a Wilcoxon signed-rank test (two-tailed) to compare the saccade choice rates of bad objects between before and after bicuculline injection (Fig. 4B and fig. S4A) and saline injection (fig. S4B).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.