Cells were seeded in six-well plates at 500 cells per well. The next day, cells were drugged at the indicated doses, and drug medium was replaced every 3 to 4 days. Approximately 1 week after treatment, plates were rinsed with PBS and fixed and stained with 0.5% (w/v) crystal violet in 6.0% (v/v) glutaraldehyde solution (Thermo Fisher Scientific) for 20 min at room temperature. Plates were rinsed in deionized water, dried overnight, and photographed the following day. Percent colony area covered by crystal violet was quantified using the ImageJ Software colony area plugin.

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