Cells were seeded in six-well plates overnight. The next day, cells were treated with the indicated amount of drug(s) or vehicle control (DMSO). Incubation time was 15 hours for etoposide or 48 hours for DRA alone or DRA with sensitizers. To prepare samples for flow cytometry, each well of cells was washed twice with ice-cold PBS and resuspended in 1X annexin V binding buffer [10 mM Hepes, 140 mM NaCl, and 2.5 mM CaCl2 (pH 7.4); BD Biosciences]. Allophycocyanin-conjugated annexin V was used to measure surface exposure of phosphatidylserine, and 7-aminoactinomycin D was used as a viability probe (BD Biosciences). Cell samples were analyzed at 20,000 counts per sample using BD FACSVantage SE.

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