Cotton gauze was ultrasonically cleaned in ethanol and water, and then coated with net-poly(MA-11-2-11-MA)-co-(MA-11-RhB). After copolymerization in a solution of MA-11-2-11-MA (5.0 mM), MA-11-RhB (0.50 mM), and ACVA (0.25 mM) at 65°C for 5 hours, the gauze was rinsed thoroughly with DI water to wash off any unreacted residue (Fig. 6B). Then, the coated cotton gauze as well as untreated cotton gauze serving as a control were taken 0.25 g of each and immersed completely in 1.0-ml S. aureus suspension (1 × 105 colony-forming units/ml). After culturing at 37°C for 24 hours, the gauzes were taken out and washed with phosphate-buffered saline (PBS) buffer solution to remove the floating S. aureus. The gauzes were then ultrasonically treated in a PBS buffer solution (2.0 ml) for 10 min to release the adherent S. aureus. The released S. aureus suspension was diluted for a series of times and cultured at 37°C for 24 hours.

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