To determine the Tt of the ELP fusion proteins, the optical turbidity at 350 nm of a 25 μM solution of the ELP fusion proteins was measured at a thermal ramp rate of 1°C/min between 4° and 60°C on a temperature-controlled ultraviolet-visible (UV-vis) spectrophotometer (Cary 300 UV-Vis, Agilent Technologies). The LCST phase transition is indicated by the sudden increase in optical turbidity, and the inflection point of the OD350 v. temperature curve was used to calculate the Tt.

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