Preparation of net-poly(MA-11-2-11-MA)-co-(MA-11-RhB) capsules
This protocol is extracted from research article:
Two-dimensional polymers with versatile functionalities via gemini monomers
Sci Adv, Nov 22, 2019; DOI: 10.1126/sciadv.aaw9120

Silica microrods and microboards were synthesized according to previous reports (39, 40). Silica microspheres were commercially purchased. Approximately 1.0 mg of each silica microparticle (i.e., microspheres, microrods, and microboards) was washed twice with DI water in a centrifugal tube. A solution (10 ml, 45°C) of MA-11-2-11-MA (5.0 mM) and MA-12-RhB (0.50 mM) was injected into the centrifugal tube under ultrasonic. The mixed solution was then cooled to room temperature, transferred to a test tube containing the initiator ACVA (25 μl, 100 mM), and degassed with nitrogen bubbling. The polymerization was carried out at 65°C for 5 hours, and the obtained microparticles were washed twice with DI water.

The capsules of net-poly(MA-11-2-11-MA)-co-(MA-11-RhB) were prepared by etching the silica microparticles with hydrofluoric acid solution (2 wt %). TEM energy dispersive spectrometry confirmed the completely removal of silica microparticles (with capsules from microrod templates as an example; fig. S9A).

The silica microrods were partially hydrolyzed when heated in aqueous solution. Therefore, one side of the silica microrod was relative more porous (fig. S9B), resulting in the capsules with a deeper contrast in TEM images (Fig. 5B and fig. S9C) and brighter fluorescence in fluorescence microscope images (Fig. 5E) on the porous side.

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