This study was designed to improve the delivery and therapeutic efficacy of extrinsic apoptotic pathway agonists in human CRC cells. We addressed this objective by (i) evaluating the potency of a multivalent DRA in a panel of CRC cell lines, (ii) performing a CRISPR-mediated knockout screen in DRA-resistant CRC cells to identify genetic drivers of DRA resistance and overcome resistance by combining DRA with sensitizers informed by the knockout screen, (iii) developing a DRA fusion to ELPs form a subcutaneously injectable gel depot for slow release of DRA into the circulation, and (iv) assessing the in vivo therapeutic efficacy of the ELPdepot-DRA formulation in combination with the best sensitizers.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.