FITC (0.39 g, 1.00 mmol) was dissolved in 20-ml anhydrous DCM under the ice bath. Then, NH2-EO4-N3 (0.24 g, 1.1 mmol) was added. The solution was allowed to slowly rise to room temperature and reacted overnight in the dark. After reaction, the solution was concentrated, and the product was purified by column chromatography. Fls-EO4-N3 was obtained as a brown oil with a yield of 41%.

1H NMR (500 MHz, DMSO): δ (ppm) 8.30 (s, 1H, Ph), 7.75 (d, 1H, Ph), 7.17 (d, 1H, Ph), 6.67 (s, 2H, Ph), 6.57 (m, 4H, Ph), 3.70 to 3.45 (m, 14H, CSNH─(CH2CH2O)3CH2─), and 3.25 (t, 2H, ─CH2─N3). MALDI-TOF (positive ion): Calcd m/z for C29H29O8N5S: 607.17; found, 608.21 (+H+).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.