MA-11-Fls was synthesized through a two-step method. FITC (0.39 g, 1.0 mmol) was dissolved in 20-ml anhydrous DCM under the ice bath, and N,N-dimethylethylenediamine (0.50 ml, 4.6 mmol) was added. The solution was allowed to slowly rise to room temperature and reacted overnight in the dark. Then, the solution was concentrated and deposited in diethyl ether three times to remove the impurities.

1H NMR (500 MHz, DMSO): δ (ppm) 8.35 (s, 1H, Ph), 7.76 (d, 1H, Ph), 7.17 (d, 1H, Ph), 6.67 (s, 2H, Ph), 6.57 (m, 4H, Ph), 3.59 (s, 2H, N─CH2CH2─NHSC), 2.40 (s, 2H, N─CH2─CH2─NHSC), 2.21 (s, 6H, N─CH3), and 10.22 and 8.00 (─OH and ─NH─, position and integral variable).

Next, above product (0.24 g, 0.5 mmol), 11-bromoundecyl methacrylate (0.16 g, 0.5 mmol) and 4-methoxyphenol (trace) were dissolved in 2-ml DMSO under nitrogen atmosphere. After reaction at 45°C for 24 hours in the dark, the solution was deposited into a mixed solvent (diethyl ether:THF = 5:1 by volume) three times to remove the impurities. MA-11-Fls was obtained as a brown-yellow solid with a yield of 32%.

1H NMR (500 MHz, DMSO): δ (ppm) 8.23 (s, 1H, Ph), 7.74 (d, 1H, Ph), 7.22 (d, 1H, Ph), 6.68 (s, 2H, Ph), 6.57 (m, 4H, Ph), 6.00 (s, 1H, CH2═C), 5.66 (s, 1H, CH2═C), 4.07 (t, 2H, ─CH2─OOC), 3.75 (t, 2H, CSNH─CH2─CH2─N), 3.52 (m, 2H, CH2CH2─N; 2H, CSNH─CH2CH2─N), 3.12 (s, 6H, N-CH3), 1.87 (s, 3H, CH3─C═C), 1.72 (m, 2H, ─CH2─CH2─OOC), 1.59 (m, 2H, ─CH2─CH2─N), and 1.20 to 1.35 [m, 14H, ─(CH2)7─]. MALDI-TOF (positive ion): Calcd m/z for C40H50O7N3S+: 716.34; found, 716.28.

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