All values are reported as means ± SEM. Continuous ratio scale data were evaluated by unpaired Student’s t test (for comparison between two samples) or analysis of variance (ANOVA) (for multiple comparisons) with post hoc analysis using Student’s t test with Bonferroni adjustments. Nonparametric analysis was performed using the Kruskal-Wallis, Kolmogorov-Smirnov, or Mann-Whitney tests. Statistical calculations were performed using GraphPad Prism version 6.0 (GraphPad Software, San Diego, CA). Statistical difference was accepted at P < 0.05, symbols are explained in the figure legend, and descriptions of the samples compared are included in the figure legend. For each experiment, n is indicated in the figure legend and refers to biological replicates, often either mice or individual wells of an experiment. Where applicable, the number of repeated experiments is indicated in the figure legend.

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