Absorbance values at 540 and 990 nm were determined along the surface of the entire gel (diameter of ≈1.5 cm) using a 1536-well plate setup in a BioTek Synergy 2 plate reader. Each grid has a finite size of ≈2 mm × 2 mm, which results in approximately 110 grids along the entire gel for which the absorbance values are obtained, leading to a dose map on the gel surface. The final absorbance value at a given grid was calculated on the basis of the method described for generating the calibration curve. The calibration curve was used to predict the radiation dose delivered to each point on the grid, and a corresponding topographical dose map over the entire gel surface is obtained. The regions that received high doses of radiation are depicted in red, and those receiving low radiation doses are depicted in blue.

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