A Leica TCS SP5 AOBS spectral confocal system was used to acquire images in the reflectance mode using a 40× water immersion lens to obtain information on the nanoparticle distribution within the gel. Z-stack images were acquired in slices of 10 μm using an argon ion laser with a 488-nm wavelength. Each plane represents a 100 μm × 100 μm field of view. Two-dimensional (2D) confocal Z stacks (≈100 slices) were stacked on ImageJ, and the 3D volume projection was obtained.

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