Freshly prepared fluorescein solution (100 μl of 0.2 mM in MilliQ water) was pipetted in a 96-well plate. Each well was exposed to a 400-nm wavelength laser with a spot size of 2 mm and a power of 180 mW for the corresponding time interval. After exposure to the laser, the fluorescence emission was measured at 515 nm. The loss in signal was normalized on the basis of the fluorescence emission of pristine fluorescein (not exposed to laser).

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