N = 4 (from one experiment) and N = 10 (from three independent experiments) replicate plates (one well per compound per plate, four fields per well, n = 1000 to 5000 mitochondria per field) were used for the primary screen and rescreen, respectively. Hits were picked on the basis of a robust Z-score cutoff of 2.5. Detailed data analysis can be found in the “Automated data analysis pipeline” section. Compounds are significantly different from control if less than or greater than 2 robust Z-scores. Data of functional assays are from N = 4 replicate plates (8 wells per compound per plate, two independent experiments). Statistical tests were performed using GraphPad Prism 7.0 (GraphPad Software Inc.)

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