Aβ(1–42) and Aβ(1–40) peptide fragments were prepared fresh as described previously (5). Briefly, hexafluoroisopropanol-treated peptide (BACHEM) was solubilized in water-free DMSO to 2.2 mM and sonicated in a bath sonicator for 30 s. Cold feeding media without B27 was added to achieve a concentration of 125 μM peptide. Peptide was incubated at 4°C for 24 hours and centrifuged at 12,000g for 10 min. The formation of oligomers was confirmed by native polyacrylamide gel electrophoresis. After determining the concentration of the oligomeric peptide in the supernatant, the oligomers were added to the mouse primary neuronal cultures at the desired concentration on DIV13 and incubated for 2 days.

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