Diffusion coefficients obtained for the different lipid in the different conditions tested were binned in groups every 10−3 μm2/s from 0 to 1.2 μm2/s. The distribution was cumulated and normalized. It was then split into three parts: slow (Da < 10−2 μm2/s), intermediate (10−2Da < 10−1 μm2/s), and fast (Da ≥ 10−1 μm2/s). The relative proportion (Pi) of the total distribution (0 ≤ Pi ≤1) of each of these parts was then calculated for each molecule inside the assembly site, outside the assembly site, and in noninfected or transfected cells. The confinement index is defined for each of the three parts as the quotient of the Pi value found inside the assembly site over the Pi value found outside the assembly site or in noninfected or transfected cells (Fig. 4).

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