Jurkat T cells (2 × 106) were microporated with 4 μg of plasmid expressing NL4.3 HIV-1 Gag.eGFP using the AMAXA system (Lonza), as previously described in (33). After microporation, Jurkat T cells were incubated in RPMI complete medium and harvested 24 hours after transfection for microscopy acquisitions as described in the following sections. For supplemental information concerning the choice of the transfected Jurkat T cells expressing Gag.eGFP, see fig. S6.

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