Fully infectious NL4.3 HIV-1 Gag-iGFP particles were prepared from the tissue culture supernatant of 293 T cells transfected using polyethyleneimine with 15 μg of pNL4.3 HIV-1 Gag-iGFP plasmid. Tissue culture supernatants were harvested 48 hours after transfection, and particles were concentrated using a Lenti-X Concentrator reagent (Clontech) according to the manufacturer’s instructions. Concentrated particles were snap-frozen and stored in aliquots at −80°C.

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