Crystallization experiments were carried out with the sitting-drop vapor diffusion method. ScoIox [0.15 μl, 54 mg/ml in 10 mM tris-HCl (pH 8.0)] was mixed with 0.15 μl of precipitant [25% (w/v) polyethylene glycol (PEG) 1500, 0.1 M succinic acid/sodium phosphate/glycine (SPG) buffer (pH 4.0)], and crystals (space group P 1 21 1) grew within several weeks at 20°C. ScoI·Cu2+ [0.1 to 0.15 μl, 47 mg/ml in 10 mM tris-HCl (pH 8.0)] was mixed with 0.15 to 0.2 μl of 20% (w/v) PEG 6000 and 0.1 M Na-citrate (pH 5.0) at 20°C. Crystals (space group P 21 21 2) grew within several weeks at 20°C. Crystals of CoxB·CuA were obtained as a side product of our attempts to crystallize the ScoI·Cu2+·CoxB complex. ScoI·Cu2+·CoxB [0.1 to 0.15 μl, 25.9 mg/ml in 5 mM Mops-NaOH (pH 7.0)] was mixed with 0.15 to 0.2 μl of 10% (w/v) PEG 1000, 10% (w/v) PEG 8000, 0.2 M potassium bromide, and 0.1 M tris-HCl (pH 7.5). Crystals of CoxB·CuA (space group P 1 21 1) grew within ~10 days at 4°C.

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