Protein concentrations were measured via the specific absorbance at 280 nm using the following extinction coefficients: CoxBSS (28,085 M−1 cm−1), apo-CoxB (27,960 M−1 cm−1), CoxB·CuA (26,950 M−1 cm−1), ScoISS (10,555 M−1 cm−1), apo-ScoI (10,430 M−1 cm−1), ScoI·Cu2+ (10,430 M−1 cm−1), ScoI·Cu2+·CoxB complex (38,390 M−1 cm−1), apo- and holo-PcuC, PcuC ΔC (8480 M−1 cm−1), and TEV-protease (32,290 M−1 cm−1).

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