Mice were housed under standard specific pathogen–free conditions, and all animal experiments were performed in accordance with protocols approved by the Animal Ethics Committee of China Pharmaceutical University. MMTV-PyMT transgenic mice on FVB background were purchased from the Jackson laboratory (#002374), and the colony was maintained in our laboratory. Male athymic BALB/c nu/nu nude mice were obtained from Qinglongshan Animal Facility (Nanjing, China). The maximal tumor sizes permitted under the approved protocols are 3 cm (length) by 3 cm (width). The clinical study was approved by the Ethics Committee of the First Affiliated Hospital of Nanjing Medical University, and written informed consents were obtained from each participant before procedure.

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